A simple, sensitive and precise RP-UPLC method for estimation of Emtricitabine has been developed and validated for determination of compounds in commercial tablet dosage form. The compounds were well separated isocratically on a ODS column using a mobile phase consisting of buffer (pH 1.9), butanol and methanol in the ratio of 70:25:5 v/v/v 0.3µl/min with PDA detector. Retention time for Emtricitabine was found to be 1.7min. The method was validated in accordance with ICH guidelines The study showed that the reverse phased liquid chromatography is sensitive and selective for detecting Emtricitabine using the single mobile phase.
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